Structure of NicX a Overall structure of NicX. Ribbon plot
Download scientific diagram | Structure of NicX a Overall structure of NicX. Ribbon plot representation of the NicX hexamer. b Overall structure of a protomer of NicX. The α-helices, β-sheets, and loops are in red, yellow, and green, respectively. Secondary structure elements of NicX are labeled. c Coordination sites of NicX-Fe(II). The 2FO-FC electron density map is contoured at 1σ, colored in blue; water 1 molecule is opposite Asp³²⁰, while water 2 molecule is opposite Ser³⁰²; green, red, blue, and purple represent carbon, oxygen, nitrogen, and Fe atoms, respectively. d The 14 Å-deep substrate-binding pocket on the NicX surface, two channels are observed. e Two channels are separated by residues His¹⁰⁵ and Glu³⁰⁸, observed in resting or NFM complex structure. f In the DHP-bound subunits, channel II was blocked by the Leu¹⁰⁴ and His¹⁰⁵ residues. from publication: Structure-guided insights into heterocyclic ring-cleavage catalysis of the non-heme Fe (II) dioxygenase NicX | Biodegradation of aromatic and heterocyclic compounds requires an oxidative ring cleavage enzymatic step. Extensive biochemical research has yielded mechanistic insights about catabolism of aromatic substrates; yet much less is known about the reaction mechanisms underlying | Dioxygenases, Iron and Pseudomonas putida | ResearchGate, the professional network for scientists.
Xingyu OUYANG, PhD Student, Bachelor of Science, Shanghai Jiao Tong University, Shanghai, SJTU, Department of Bioinformatics and Biological Statistics
Liu GONGQUAN, Shanghai Jiao Tong University, Shanghai, SJTU, Department of Bioengineering
Liu GONGQUAN, Shanghai Jiao Tong University, Shanghai, SJTU, Department of Bioengineering
Xingyu OUYANG, PhD Student, Bachelor of Science, Shanghai Jiao Tong University, Shanghai, SJTU, Department of Bioinformatics and Biological Statistics
Ping XU, Professor, PhD, Virginia Commonwealth University, Virginia, VCU, Philips Institute of Oral and Craniofacial Molecular Biology
PDF) Structure-guided insights into heterocyclic ring-cleavage catalysis of the non-heme Fe (II) dioxygenase NicX
Structure of NicX a Overall structure of NicX. Ribbon plot
Hongzhi TANG, Professor (Full), Ph.D., Shanghai Jiao Tong University, Shanghai, SJTU, State Key Laboratory of Microbial Metabolism
Xingyu OUYANG, PhD Student, Bachelor of Science, Shanghai Jiao Tong University, Shanghai, SJTU, Department of Bioinformatics and Biological Statistics
Liu GONGQUAN, Shanghai Jiao Tong University, Shanghai, SJTU, Department of Bioengineering
Structure of NicX a Overall structure of NicX. Ribbon plot
